Platelet-derived Growth Factor-BB
human, recombinant, Saccharomyces cerevisiae
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 32 kDa
Accession number: P01127
Accession number: P01127
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilized (PDGF-BB was lyophilized from 0.2 μm filtered 20 mM sodium acetate in absence of any carrier protein)
Solubility: It is recommended to reconstitute the lyophilized PDGF-AB in sterile bidest H2O not less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: ED50: < 1 ng/ml, corresponding to a specific activity of 1 x 106 IU/mg, calculated by the dosedependent proliferation of murine BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake).
PDGF is a mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by Interleukin-1, Interleukin-6, TNF-α, TGF-β and EGF. Recombinant Human Platelet Derived Growth Factor-BB is a homodimeric, glycosylated polypeptide chain containing 109 amino acids and having a molecular mass of 32 kDa. PDGF-BB is purified by proprietary chromatographic techniques.
Wollina et al. (2004) Topical PDGF-BB results in limited healing in a patient with Werner's syndrome and chronic leg ulcers. J. Wound. Care. 13:415.
Cao et al. (2004) PDGF-BB induces intratumoral lymphangiogenesis and promotes lymphatic metastasis. Cancer Cell. 6:333.
Cartel et al. (2005) Abrogation of apoptosis through PDGF-BB-induced sulfated glycosaminoglycan synthesis and secretion. Am. J. Physiol. Lung. Cell. Mol. Physiol. 288:L285.
Hao et al. (2004) Angiogenic and cardiac functional effects of dual gene transfer of VEGF-A165 and PDGF-BB after myocardial infarction. Biochem. Biophys. Res. Commun. 322:292.
Imanishi et al. (2004) Herb medicine Inchin-ko-to (TJ-135) regulates PDGF-BB-dependent signaling pathways of hepatic stellate cells in primary culture and attenuates development of liver fibrosis induced by thioacetamide administration in rats. J. Hepatol. 41:242.
Ricci et al. (2004) Embryonic mouse testis development: role of platelet derived growth factor (PDGF-BB). J. Cell. Physiol. 200:458.