Platelet-derived Growth Factor-AB
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 26 kDa
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Solubility: It is recommended to reconstitute the lyophilised PDGF-AB in sterile bidest H2O not less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: ED50: < 1 ng/ml, corresponding to a specific activity of 1 x 106 IU/mg, calculated by the dosedependent proliferation of murine BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake).
PDGF is a mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by Interleukin-1, Interleukin-6, TNF-α, TGF-β and EGF. Recombinant Human PDGF-AB is a heterodimeric, non-glycosylated, polypeptide chain containing 234 amino acids consisting of 13.3 kDa alpha-chain and 12.2 kDa beta-chain having a total molecular mass of 25.5 kDa. Recombinant PDGF-AB is purified by proprietary chromatographic techniques.
Endotoxin: Less than 0.1 ng/μg (IEU/μg) of PDGF-AB.
Donati et al. (2002) Platelet activation and PDGF-AB release during dialysis. Int. J. Artif. Organs. 25:1128.
Affleck et al. (2002) PDGF (BB) increases myocardial production of VEGF: shift in VEGF mRNA splice variants after direct injection of bFGF, PDGF (BB), and PDGF (AB). J. Surg. Res. 107:203.
Lachapelle et al. (2002) Fibroblast growth factor-2 (FGF-2) and platelet-derived growth factor AB (PDGF AB) promote adult SVZderived oligodendrogenesis in vivo. Mol. Cell. Neurosci. 20:390.
Loot et al. (2002) Fibroblasts derived from chronic diabetic ulcers differ in their response to stimulation with EGF, IGF-I, bFGF and PDGF-AB compared to controls. Eur. J. Cell. Biol. 81:153.
Cianciolo et al. (2001) Intra- and post-dialytic platelet activation and PDGF-AB release: cellulose diacetate vs polysulfone membranes. Nephrol. Dial. Transplant. 16:1222.