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PDGF-AA

Platelet-derived Growth Factor-AA

human, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-494 10 μg 253,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 29 kDa

Accession number: P04085

Accession number: P04085

Purity: > 95 % (SDS-PAGE, RP-HPLC)

Form: lyophilized

Solubility: It is recommended to reconstitute the lyophilized PDGF-AA in sterile bidest H2O not less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Activity: ED50: < 1 ng/ml, corresponding to a specific activity of 1 x 106 IU/mg, calculated by the dosedependent proliferation of murine BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake).

Description:
PDGF is a mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by Interleukin-1, Interleukin-6, TNF-α, TGF-β and EGF. Recombinant Human Platelet Derived Growth Factor-AA is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 125 amino acids and having a total molecular mass of 28.51 kDa. Recombinant PDGF-AA is purified by proprietary chromatographic techniques.

Endotoxin: Less than 0.1 ng/μg (IEU/μg) of PDGF-AA.

Selected References:
Ingram et al. (2004) IL-13 and IL-1beta promote lung fibroblast growth through coordinated up-regulation of PDGF-AA and PDGF-R alpha. FASEB J. 18:1132.
Vinader et al. (2003) Influence of hormones and growth factors on lens protein composition: the effect of dexamethasone and PDGFAA. Mol. Vis. 9:723.
Diemel et al. (2003) Role for TGF-beta1, FGF-2 and PDGF-AA in a myelination of CNS aggregate cultures enriched with macrophages. J. Neurosci. Res. 74:858.
Kastin et al. (2003) Different mechanisms influencing permeation of PDGF-AA and PDGF-BB across the blood-brain barrier. J. Neurochem. 87:7.
Inkinen et al. (2003) Expression of TGF-beta and PDGF-AA antigens and corresponding mRNAs in cytomegalovirus-infected rat kidney allografts. Transplant. Proc. 35:804.
Mason et al. (2002) A2B5+ and O4+ Cycling progenitors in the adult forebrain white matter respond differentially to PDGF-AA, FGF-2, and IGF-1. Mol. Cell. Neurosci. 20:30.