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NT-3 - NGF-2

Neurotrophin-3, Nerve Growth Factor 2

human, recombinant, E. coli

Product Cat. No. Amount Price (EUR) Buy / Note
NT-3 - NGF-2 PR-493 10 μg 253,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 14 kDa

Accession number: P20783

Accession number: P20783

Purity: > 95 % (SDS-PAGE, RP-HPLC)

Form: lyophilized

Solubility: It is recommended to reconstitute the lyophilized NT-3 in sterile bidest H2O not less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Activity: ED50: < 20-50 ng/ml, determined by the dosedependent induction of choline acetyl transferase in rat basal forebrain primary septal culture.

NT-3 is a neurotrophic factor involved in regulating the survival of visceral and proprioceptive sensory neurons. It is closely homologous to Nerve Growth Factor beta (NGF-b) and BDNF. NT-3 is found in neurons of the central nervous system. NT-3 is expressed also in muscles and its expression is downregulated in denervated muscles. Many human gliomas express and secrete NT-3 into the conditioned medium. Recombinant human NT-3 produced in E. coli is a single, non-glycosylated polypeptide chain containing 119 amino acids and having a molecular mass of 13.6 kDa. Recombinant human NT-3 is purified by proprietary chromatographic techniques.

Endotoxin: Less than 0.1 ng/μg (IEU/μg) of NT-3.

Selected References:
Chaudhry et al. (2000) Tolerability of recombinant-methionyl human neurotrophin-3 (r-metHuNT3) in healthy subjects. Muscle Nerve. 23:189.
Suenaga et al. (1998) Renaturation of recombinant human neurotrophin-3 from inclusion bodies using a suppressor agent of aggregation. Biotechnol. Appl. Biochem. 28:119.
Kaisho et al. (1998) Tissue and cell type specificity of the human neurotrophin-3 promoter region in transgenic mice. Brain. Res. Mol. Brain. Res. 59:114.
Hui et al. (1996) Human neurotrophin-3: a one-step peptide mapping method and complete disulfide characterization of the recombinant protein. J. Protein. Chem. 15:351.