Keratinocyte Growth Factor 1
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 19 kDa
Accession number: P21781
Accession number: P21781
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Solubility: It is recommended to reconstitute the lyophilised KGF in bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: ED50: < 10 ng/ml, corresponding to a specific activity 105 IU/mg, stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake).
Keratinocyte growth factor (KGF) is a paracrine-acting, epithelial mitogen produced by cells of mesenchymal origin. Keratinocyte growth factor-1 (KGF-1) is the seventh member of the fibroblast growth factor family. It is produced by mesenchymal cells such as fibroblasts and upregulated in a variety of hyperplastic tissues. It is thought to play an important role in the paracrine growth control of normal epithelial cells. KGF stimulates the proliferation of primary and secondary human keratinocytes to the same extent as EGF. Recombinant human KGF-1 (FGF-7) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 19 kDa. Recombinant KGF-1 is purified by proprietary chromatographic techniques.
Marchand-Adam et al. (2005) KGF expression by fibroblasts in pulmonary fibrosis: poor response to interleukin-1 beta. Am. J. Respir. Cell. Mol. Biol. 32:470.
Zang et al. (2004) KGF-induced motility of breast cancer cells is dependent on Grb2 and Erk1,2. Clin. Exp. Metastasis. 21:437.
Sharma et al. (2004) Epidermal and hepatocyte growth factors, but not keratinocyte growth factor, modulate protein kinase Calpha translocation to the plasma membrane through 15 (S)- hydroxyeicosatetraenoic acid synthesis. J. Biol. Chem. 280:7917.
Washizawa et al. (2004) Comparative effects of glucagon-like peptide-2 (GLP-2), growth hormone (GH), and keratinocyte growth factor (KGF) on markers of gut adaptation after massive small bowel resection in rats. JPEN J. Parenter. Enteral. Nutr. 28:399.
Orskov et al. (2005) GLP-2 stimulates colonic growth via KGF, released by subepithelial myofibroblasts with GLP-2 receptors. Regul. Pept. 124:105.