Insulin Like Growth Factor Binding Protein 1

human, recombinant, mouse myeloma (NSO) cells

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PR-456 20 μg 253,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 29 kDa

Accession number: P08833

Accession number: P08833

Purity: > 95 % (SDS-PAGE, RP-HPLC)

Form: lyophilized (IGFBP-1 was lyophilized after dialysis against 10 mM HCl)

Solubility: It is recommended to reconstitute the lyophilized IGFBP-1 in sterile bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Activity: IC50: {tab??} 100 ng/ml (Inhibition of IGF-I stimulated proliferation of NUC-1 cells). IC50 (1% serum): {tab??} 300 ng/ml (Inhibition of seruminduced stimulation of DNA synthesis in chick embryo fibroblast primary cultures).

IGFBP-1 is one of the six homologous proteins that specifically bind insulin-like growth factors and modulate their mitogenic and metabolic actions. The function of this protein is not completely defined. However, several studies demonstrate that it inhibits IGF binding to cell surface receptors and thereby inhibits IGF-mediated mitogenic and cell metabolic actions. Insulin Like Growth Factor Binding Protein modulate IGF activities by increasing their plasma half lives and by inhibiting or promoting the interactions of IGF with receptors on certain target cells. In addition these binding proteins provide a reservoir for IGF in pericellular spaces. Some IGFBPs also have stimulating effects in vitro and some may inhibit the growth of cells. Recombinant human Insulin Like Growth Factor Binding Protein 1 is a single glycosylated polypeptide chain containing 218 amino acids and having a molecular mass of 28.806 kDa. The IGFBP-1 is purified by proprietary chromatographic techniques.

Endotoxin: Less than 0.1 ng/μg (IEU/μg) of IGFBP-1.

Selected References:
Liu et al. (1991) IGFBP-1, an insulin like growth factor binding protein, is a cell growth inhibitor. Biochem. Biophys. Res. Comm. 174:673.
Schuller et al. (1993) Localization of the epitope of a monoclonal antibody against human insulin-like growth factor binding protein-1, functionally interfering with insulin-like growth factor binding. Growth Regul. 3:32.
Kajimura et al. (2005) From the Cover: Insulin-like growth factor-binding protein-1 (IGFBP-1) mediates hypoxia-induced embryonic growth and developmental retardation. Proc. Natl. Acad. Sci. USA 102:1240.
Lathi et al. (2004) Dose-Dependent Insulin Regulation of IGFBP-1 in Human Endometrial Stromal Cells is Mediated by Distinct Signaling Pathways. J. Clin. Endocrinol. Metab.
Hills et al. (2004) Regulation of human villous trophoblast by insulinlike growth factors and insulin-like growth factor-binding protein-1. J. Endocrinol. 183:487.
Davies et al. (2004) Progesterone inhibits insulin-like growth factor binding protein-1 (IGFBP-1) production by explants of the Fallopian tube. Mol. Hum. Reprod. 10:935.
Skjaerbaek et al. (2004) Free IGF-I, IGFBP-1, and the binary complex of IGFBP-1 and IGF-I are increased during human pregnancy. Horm. Res. 62:215.