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Interferon Regulatory Factor-3 human

human, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-451 20 μg 253,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA)

Shelf Life: 12 months

Molecular Weight: 13 kDa

Accession number: Q14653

Accession number: Q14653

Purity: > 95 % (SDS-PAGE, RP-HPLC)

Form: liquid (Supplied in PBS pH 7.4)

Members of the Interferon regulatory factor (IRF) family regulate gene expression critical to immune response, hemopoiesis, and proliferation. IRF-3 is a member of the IRF family, and is distinct from other family members. Its transcriptional activity is regulated solely by posttranslational modifications. It plays a crucial role in activation of innate immunity and inflammation in response to viral infection. IRF-3 mediates interferon-stimulated response element (isre) promoter activation. Functions as a molecular switch for antiviral activity. Dsrna generated during the course of an viral infection leads to IRF3 phosphorylation on the c-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with creb binding protein (crebbp) to form dsrna-activated factor 1 (draf1), a complex which activates the transcription of genes under the control of isre. The complex binds to the ie and prdiii regions on the ifn-alpha and ifn-beta promoters respectively. IRF-3 does not have any transcription activation domains. IRF-3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 111 amino acids (1-112) and having a molecular mass of 13 kDa. The Interferon Regulatory Factor-3 is purified by proprietary chromatographic techniques.

Selected References:
Hiscott (2007) Triggering the innate antiviral response through IRF-3 activation. J. Biol. Chem. 282:15325.
Hiscott et al. (2005) IRF-3 releases its inhibitions. Structure 13:1269.
Preston et al. (2001)Activation of interferon response factor-3 in human cells infected with herpes simplex virus type 1 or human cytomegalovirus. J. Virol. 75:8909.