Insulin Like Growth Factor I, IGF-1
rat, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 8 kDa
Accession number: P08025
Accession number: P08025
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilized (IGF-I was lyophilized after dialysis against 100 mM acetic acid)
Solubility: It is recommended to reconstitute the lyophilized IGF-I in sterile bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: EC50: < 30 ng/ml, calculated by stimulation of protein synthesis in rat L6 myoblasts, and < 10 ng/ml calculated by type 1 IGF receptor binding assay.
IGF is a well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor II, which is a major fetal growth factor. Recombinant rat IGF-I produced in E. coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.687 kDa. Recombinant IGF-I is purified by proprietary chromato- graphic techniques.
Endotoxin: Less than 0.1 ng/μg (IEU/μg) of IGF-I.
Shimatsu et al. (1987) Mosaic evolution of the insulinlike growth factors. Organization, sequence, and expression of the rat insulin-like growth factor I gene. J. Biol. Chem. 262:7894.
Upton et al. (1996) Characterization of serum-derived and recombinant rat IGF-I and their use for measuring true concentrations of IGF-I in rat plasma. J. Endocrinol. 149:379.
Szpechcinski et al. (2004) Presence of MHC-I in rat glioma cells expressing antisense IGF-I-receptor RNA. Rocz. Akad. Med. Bialymst. 49:98.
Oberbauer et al. (1995) Growth hormone and IGF-I stimulate cell function in distinct zones of the rat epiphyseal growth plate. Connect. Tissue Res. 31:189.
Matsakas et al. (2004) Effect of voluntary exercise on the expression of IGF-I and androgen receptor in three rat skeletal muscles and on serum IGF-I and testosterone levels. Int. J. Sports Med. 25:502.
Thorne et al. (2004) Delivery of insulin-like growth factor-I to the rat brain and spinal cord along olfactory and trigeminal pathways following intranasal administration. Neuroscience. 127:481.
Saile et al. (2004) IGF-I induces DNA synthesis and apoptosis in rat liver hepatic stellate cells (HSC) but DNA synthesis and proliferation in rat liver myofibroblasts (rMF). Lab. Invest. 84:1037.
Scharf et al. (2004) Insulin-like growth factor (IGF)-binding protein-1 is highly induced during acute carbon tetrach loride liver injury and potentiates the IGF-I-stimulated activation of rat hepatic stellate cells. Endocrinology. 145:3463.