Insulin Like Growth Factor I, IGF-1
murine, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 8 kDa
Accession number: P05017
Accession number: P05017
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilized (IGF-I was lyophilized after dialysis against PBS)
Solubility: It is recommended to reconstitute the lyophilized IGF-I in sterile bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: EC50: < 1.0 ng/ml, corresponding to a specific activity of 1 x 106 U/mg, calculated by the dosedependent proliferation of murine BALB
IGF is a well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor II, which is a major fetal growth factor. Recombinant murine IGF-I produced in E. coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6 kDa. Recombinant IGF-I is purified by proprietary chromato- graphic techniques.
Endotoxin: Less than 0.1 ng/μg (IEU/μg) of IGF-I.
Adhami et al. (2004) Oral consumption of green tea polyphenols inhibits insulin-like growth factor-I-induced signaling in an autochthonous mouse model of prostate cancer. Cancer Res. 64:8715.
Fabian et al. (2004) Inhibitory effect of IGF-I on induced apoptosis in mouse preimplantation embryos cultured in vitro. Theriogenology. 61:745.
Herman et al. (2004) Insulin-like growth factor-I governs submucosal growth and thickness in the newborn mouse ileum. Pediatr. Res. 55:507.
Kurzawa et al. (2002) Evaluation of mouse preimplantation embryos exposed to oxidative stress cultured with insulin-like growth factor I and II, epidermal growth factor, insulin, transferrin and selenium. Reprod. Biol. 2:143.
Wang et al. (2004) Development of leydig cells in the insulin-like growth factor-I (igf-I) knockout mouse: effects of igf-I replacement and gonadotropic stimulation. Biol. Reprod. 70:632.
Nahm et al. (2003) Insulin-like growth factor-I improves cerebellar dysfunction but does not prevent cerebellar neurodegeneration in the calcium channel mutant mouse, leaner. Neurobiol. Dis. 14:157.
Kubota et al. (2008) Low-dose GH supplementation reduces the TLR2 and TNF-α expressions in visceral fat. Biochemical and Biophysical Research Communications 368:81-87.