rat, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 22 kDa
Accession number: P01244
Accession number: P01244
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Solubility: It is recommended to reconstitute the lyophilized GH in sterile bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
The Growth Hormone (GH) is a polypeptide that is secreted by the adenohypophysis. Growth hormone, also known as somatotropin, stimulates mitosis, cell differentiation and cell growth. Species-specific growth hormones have been synthesized. GH augments the cytolytic activity of T-cells, antibody synthesis, and granulocyte differentiation induced by GM-CSF. GH also enhances production of TNF-alpha, generation of superoxide anions from peritoneal macrophages, and natural killer activity. Recombinant Rat GH produced in E. coli is a single, non-glycosylated, polypeptide chain containing having a molecular mass of 21.94 kDa. Rat-GH is purified by proprietary chromatographic techniques.
Endotoxin: Less than 0.1 ng/μg (IEU/μg) of GH.
Flint et al. (1993) Influence of growth hormone deficiency on growth and body composition in rats: site-specific effects upon adipose tissue development. J. Endocrinol. 137:203.
Veyrat-Durebex et al. (2004) Internalization and trafficking of the human and rat growth hormone-releasing hormone receptor. J. Cell. Physiol. 203:335.
Hajdu et al. (2003) Sleep of transgenic mice producing excess rat growth hormone. Am. J. Physiol. Regul. Integr. Comp. Physiol. 282:R70.
Spodsberg et al. (2001) Characteristics and structural requirements of apical sorting of the rat growth hormone through the O-glycosylated stalk region of intestinal sucrase-isomaltase. J. Biol. Chem. 276:46597.
Cabanillas et al. (2001) T3-activation of the rat growth hormone gene is inhibited by a zinc finger/homeodomain protein. Mol. Cell. Endocrinol. 181:131.
Johansen et al. (2000) Comparison of methods of analysis of body composition in hypophysectomized rats treated with rat growth hormone. Growth Horm. IGF Res. 10:342.
Nass et al. (2000) Intracerebroventricular administration of the rat growth hormone (GH) receptor antagonist G118R stimulates GH secretion: evidence for the existence of short loop negative feedback of GH. J. Neuroendocrinol 12:1194.