Placental Growth Hormone-22K
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 22 kDa
Accession number: P01242
Accession number: P01242
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilised (Placental Growth Hormone protein was lyophilised from a 1 mg/ml solution with 0.0045 mM NaHCO3 and pH 8.0)
Solubility: It is recommended to reconstitute and dilute the lyophilised Placental-GH in 0.4% NaHCO3 adjusted to pH 8.0, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Placental growth hormone, or growth hormone variant (GH-V), is a member of the growth hormone gene family. Its physiologic role during pregnancy remains undefined. Although extensive work has been done characterizing the signaling pathways activated by hGH, the signaling pathways which are activated by GH-V have not been characterized. Human placental GH (GH-22K) replaces pituitary GH during pregnancy. GH-22K is correlated to serum IGF-I in normal pregnancies and in pregnancies complicated by fetoplacental disorders. Recombinant Placental Human GH-22K produced in E. coli is a single, non-glycosylated, polypeptide chain containing 192 amino acids and having a molecular mass of 22.367 kDa. GH-22K has dimished lactogenic (prolactin receptor mediated) activity characteristic to pituitary GHs. Placental Growth Hormone is purified by proprietary chromatographic techniques.
Endotoxin: Less than 0.1 ng/μg (IEU/μg) of GH.
Baviera et al. (2004) Placental growth hormone in Down's syndrome screening. J. Matern. Fetal. Neonatal. Med. 16:241.
Chellakooty et al. (2004) A longitudinal study of intrauterine growth and the placental growth hormone (GH)-insulin-like growth factor I axis in maternal circulation: association between placental GH and fetal growth. J. Clin. Endocrinol. Metab. 89:384.
Fuglsang et al. (2004) Placental growth hormone during pregnancy in a growth hormone deficient woman with type 1 diabetes compared to a matching diabetic control group. Growth Horm. IGF Res. 14:66.
Barbour et al. (2004) Human placental growth hormone increases expression of the p85 regulatory unit of phosphatidylinositol 3-kinase and triggers severe insulin resistance in skeletal muscle. Endocrinology 145:1144.
Fuglsang et al. (2003) Human placental growth hormone, insulin-like growth factor I and -II, and insulin requirements during pregnancy in type 1 diabetes. J. Clin. Endocrinol. Metab. 88:4355.