Pituitary Growth Hormone
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 22 kDa
Accession number: P01241
Accession number: P01241
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilized (Pituitary Growth Hormone protein was lyophilized from a 1 mg/ml solution with 0.0045 mM NaHCO3 and pH 11.0)
Solubility: It is recommended to reconstitute and dilute the lyophilized GH in 0.4% NaHCO3 adjusted to pH 11.0, which can then be further diluted to other aqueous solutions. It is recommended when diluting to below 10 μg/ml that the dilution solution will contain carrier protein (BSA or other) to avoid non-specific absorption of the hGH to plastic tubes.
Activity: 3 U/mg
The 22 kDa human GH (GH-22K) is produced in the pituitary by alternative splicing of GH mRNA. Pituitary growth hormone (GH) plays diverse roles in the promotion of cell growth and metabolism. GH has been shown to influence the development of the immune organ and the function of immune cells. The binding of GH to its receptor causes dimerization of two growth hormone receptors (GHR), which, in turn, initiates the signal transduction in the cell. Recombinant human Pituitary Growth Hormone produced in E. coli is a single, non-glycosylated, polypeptide chain containing 192 amino acids and having a molecular mass of 22.4 kDa. Pituitary Growth Hormone is purified by proprietary chromatographic techniques.
Endotoxin: Less than 0.1 ng/μg (IEU/μg) of GH.
Ye et al. (2003) Cloning of Novel Pituitary Growth Hormone Gene from Rhinopithecus roxellanae. Yi Chuan 25:291.
Ezzat et al. (2005) The Zinc Finger Ikaros Transcription Factor Regulates Pituitary Growth Hormone and Prolactin Gene Expression Through Distinct Effects on Chromatin Accessibility. Mol. Endocrinol. 19:1004.
Lora et al. (2005) Synergistic role for pituitary growth hormone in the regulation of hepatic estrogen and progesterone receptors and vitellogenesis in female freshwater turtles, Chrysemys picta. Gen. Comp. Endocrinol. 140:25.
Xu et al. (2004) Intermittent hypoxia causes a suppressed pituitary growth hormone through somatostatin. Neuro. Endocrinol. Lett. 25:361.
Morel et al. (2004) Ectopic acromegaly: localization of the pituitary growth hormone-releasing hormone producing tumor by In-111 pentetreotide scintigraphy and report of two cases. Clin. Nucl. Med. 29:841.
Maniou et al. (2004) Episodic molecular evolution of pituitary growth hormone in Cetartiodactyla. J. Mol. Evol. 58:743.