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FGF-2 - bovine

Fibroblast Growth Factor, basic

bovine, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-417 50 μg 253,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 17 kDa

Accession number: P03969

Accession number: P03969

Purity: > 95 % (SDS-PAGE, RP-HPLC)

Form: lyophilised (Recombinant FGF-2 was lyophilised from a 1 mg/ml solution containing 1% BSA)

Solubility: It is recommended to reconstitute the lyophilised FGF in sterile bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.

Activity: ED50: < 0.1 ng/ml, corresponding to a specific activity of 2.4 x 106 Units/mg, measured in a mitogenic assay using quiescent NR6R-3T3 fibroblasts.

FGF-2 is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor. Recombinant Bovine FGF-2 (FGF-b) produced in E. coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 17.48 kDa. The rbFGF-2 is purified by proprietary chromatographic techniques.

Endotoxin: Less than 0.1 ng/μg (IEU/μg) of FGF.

Selected References:
Talbot et al. (2004) Comparison of colony-formation efficiency of bovine fetal fibroblast cell lines cultured with low oxygen, hydrocortisone, L-carnosine, bFGF, or different levels of FBS. Cloning Stem Cells 6:37.
Neuvians et al. (2004) Vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF) expression during induced luteolysis in the bovine corpus luteum. Mol. Reprod. Dev. 67:389.
Berisha et al. (2004) Expression and localization of fibroblast growth factor (FGF) family members during the final growth of bovine ovarian follicles. Mol. Reprod. Dev. 67:162.
Neuvians et al. (2004) Involvement of pro-inflammatory cytokines, mediators of inflammation, and basic fibroblast growth factor in prostaglandin F2alpha-induced luteolysis in bovine corpus luteum. Biol. Reprod. 70:473.
Saito et al. (2003) Generation of cloned calves and transgenic chimeric embryos from bovine embryonic stem-like cells.Biochem. Biophys. Res. Commun. 309:104.