Ciliary Neurotrophic Factor
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 23 kDa
Accession number: P26441
Accession number: P26441
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilized (CNTP was lyophilized from a 1 mg/ml solution containing 5 mM sodium phosphate buffer pH 7.5 and 5 mM NaCl)
Solubility: It is recommended to reconstitute the lyophilized BNP in sterile bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: ED50: < 2 ng/ml, corresponding to a specific activity of 5 x 105 IU/mg, as determined by the dosedependant stimulation of TF-1 cells.
Ciliary neurotrophic factor (CNTF) belongs to the gp130 family of cytokines. This family also includes leukemia inhibitory factor, interleukin (IL)-6, IL-11, oncostatin M, cardiotrophin-1, and the newly identified cardiotrophin-like cytokine. Among its many functions, CNTF enhances the survival of neuronal cells and has been investigated as a therapeutic agent for motor neuron disease. CNTF, like FGF acidic, FGF basic, and PD-ECGF (platelet-derived endothelial cell growth factor), does not possess a signal sequence that would allow secretion of the factor by classical secretion pathways (endoplasmatic reticulum/Golgi system). The mechanism underlying the release of CNTF is still unknown. Recombinant human CNTF produced in E. coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 22.7 kDa. Recombinant CNTF is purified by proprietary chromatographic techniques.
Man et al. (2003) Solution structure of the C-terminal domain of the ciliary neurotrophic factor (CNTF) receptor and ligand free associations among components of the CNTF receptor complex. J. Biol. Chem. 278:23285.
Setoguchi et al. (2004) Nuclear export of OLIG2 in neural stem cells is essential for ciliary neurotrophic factor-induced astrocyte differentiation. J. Cell. Biol. 166:963.
McCallister et al. (2004) Regeneration along intact nerves using nerve growth factor and ciliary neurotrophic factor. J. Reconstr. Microsurg. 20:473.
Calcutt et al. (2004) Prevention of sensory disorders in diabetic Sprague-Dawley rats by aldose reductase inhibition or treatment with ciliary neurotrophic factor. Diabetologia 47:718.
Bongioanni et al. (2004) Ciliary neurotrophic factor (CNTF) for amyotrophic lateral sclerosis/motor neuron disease. Cochrane Database Syst. Rev. (3):CD004302.
Kimata (2004) Ciliary neurotrophic factor preferentially enhances spontaneous IgE production by B cells from atopic patients. Neuropeptides 38:92.