Nerve Growth Factor β
human, recombinant, Chinese hamster ovary (CHO) cells
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 26.5 kDa
Accession number: P01138
Accession number: P01138
Purity: > 95 % (SDS-PAGE, SEC-HPLC)
Form: lyophilized (NGFβ was lyophilized from a 0.2 μm filtered solution in 20 mM Tris-Hcl pH 8.0 and 0.15M NaCl)
Solubility: It is recommended to reconstitute the lyophilized NGFβ in sterile bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
Activity: ED50: < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg, calculated by its ability to stimulate chick E9 DRG neurite outgrowth.
NGFβ produced in CHO cells is a non covalently disulfide linked homodimer, glycosylated, polypeptide chain containing 2 identical 118 amino acids and having a molecular mass of 26.5 kDa. Nerve Growth Factor is the first of a series of neurotrophic factors that were found to influence the growth and differentiation of sympathetic and sensory neurons. It is comprised of α-, β,-, and γ-subunits. The β-subunit is responsible for its growth stimulating activity. The synthesis of NGF in astrocytes is enhanced by various other cytokines: IL-1, TNFα, PDGF and TGFβ. NGF influences development differentiation, chemotaxis and mediator release of inflammatory cells as well as fibroblast activation through a complex network influenced by other pro-inflammatory cytokines. NGF is increased in biological fluids of several allergic, immune and inflammatory diseases.
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ser-Ser-His-Pro.
Bonini et al. (2003) Nerve growth factor: neurotrophin or cytokine? Int. Arch. Allergy Immunol. 131:80.
Yao et al. (2004) The effects of hypothyroidism on nerve growth factor and norepinephrine concentrations in weight-bearing and nonweight- bearing bones of rats. J. Musculoskelet. Neuronal. Interact. 4:319.
Suzuki et al. (2004) Neurotrophin-3-induced production of nerve growth factor is suppressed in Schwann cells exposed to high glucose: involvement of the polyol pathway. J. Neurochem. 91:1430.
Okada et al. (2004) Nerve growth factor stimulates MMP-2 expression and activity and increases invasion by human pancreatic cancer cells. Clin. Exp. Metastasis 21:285.
Mitsuma et al. (2004) Wide range of lineages of cells expressing nerve growth factor mRNA in the nerve lesions of patients with vasculitic neuropathy: an implication of endoneurial macrophage for nerve regeneration. Neuroscience 129:109.
Gigliozzi et al. (2004) Nerve growth factor modulates the proliferative capacity of the intrahepatic biliary epithelium in experimental cholestasis. Gastroenterology 127:1198.