Bone Morphogenetic Protein-4
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 13 kDa
Accession number: P12644
Accession number: P12644
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilized (BMP-4 was lyophilized from a 0.2 μm filtered concentrated (1mg/ml) solution in 30% Acetonitrile and 0.1% TFA)
Solubility: It is recommended to reconstitute the lyophilized BMP-4 in sterile H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommeded to add 0.1% HSA or BSA.
Molecular standard for reducing and non-reducing gels. Antibody preparation for BMP-4 monomer.
Bone morphogenetic proteins (BMPs) are key regulators of bone formation. Bone morphogenetic protein 4, BMP4, plays also an important role in the development of various organs including the lungs. Little is known regarding the regulation of Bmp4 gene expression in any organ. In the lung, indirect evidence indicates that NKX2.1, a homeodomain transcriptional factor with a demonstrated role in lung morphogenesis, may be a potential upstream regulator of Bmp4 gene expression. Hematopoietic cytokines and bone morphogenetic protein-4 (BMP-4) have been shown to modulate the proliferative and differentiative potential of definitiv human fetal, neonatal, and adult hematopoietic progenitors, in addition to their role in early hematopoietic development. Recombinant human BMP-4 produced in E. coli is a monomeric, non-glycosylated, polypeptide chain containing 116 amino acids and having a molecular mass of 13 kDa. BMP-4 is purified by proprietary chromatographic techniques.
Pang et al. (2004) Effect of recombinant human bone morphogenetic protein-4 dose on bone formation in a rat calvarial defect model. J. Periodontol. 75:1364.
Shimizu et al. (2004) Differential expression of bone morphogenetic protein 4-6 (BMP-4, -5, and -6) and growth differentiation factor-9 (GDF-9) during ovarian development in neonatal pigs. Domest. Anim. Endocrinol. 27:397.
Peng et al. (2004) Development of a self-inactivating tet-on retroviral vector expressing bone morphogenetic protein 4 to achieve regulated bone formation. Mol. Ther. 9:885.
Sasaki et al. (2004) Sonic hedgehog and bone morphogenetic protein 4 expressions in the hindgut region of murine embryos with anorectal malformations. J. Pediatr. Surg. 39:170.
Zhu et al. (2004) NKX2.1 regulates transcription of the gene for human bone morphogenetic protein-4 in lung epithelial cells. Gene. 327:25.
Wahl et al. (2004) Transcriptome analysis of early chondrogenesis in ATDC5 cells induced by bone morphogenetic protein 4. Genomics. 83:45.
Chadwick et al. (2003) Cytokines and BMP-4 promote hematopoietic differentiation of human embryonic stem cells. Blood. 102:906.
Markowski et al. (2011) BMP4 increases expression of HMGA2 in mesenchymal stem cells. Cytokine 56 (3):811.