Stem Cell Factor
rat, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 18.4 kDa
Accession number: P21581
Accession number: P21581
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilized (CSF was lyophilized from 0.02% NaHCO3)
Solubility: It is recommended to reconstitute the lyophilized SCF in sterile bidest H2O not less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: ED50: < 10 ng/ml, corresponding to a specific activity of 105 IU/mg, determined by the dosedependent stimulation of the proliferation of human MO7e cells.
Stem Cell Factor (SCF) plays an important role in hematopoiesis and survival, proliferation, and differentiation of mast cells, melanocytes, and germ cells. SCF mediates its biological effects by binding to and activating a receptor tyrosine kinase designated c-kit or SCF receptor (SCFR). SCF is also known as mast cell growth factor (MCGF), steel (Sl) factor (SLF), or kit ligand (KL). Recombinant rat SCF produced in E. coli is a single, non-glycosylated polypeptide chain containing 165 (26-189)amino acids.
Amino Acid Sequence: MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
Barreiro et al. (2004) Ghrelin inhibits the proliferative activity of immature Leydig cells in vivo and regulates stem cell factor messenger ribonucleic acid expression in rat testis. Endocrinology. 145:4825.
Wang et al. (2004) Survivin expression in rat testis is upregulated by stem-cell factor. Mol. Cell. Endocrinol. 218:165.
Huang et al. (2001) Stem cell factor and insulin-like growth factor-I stimulate luteinizing hormone-independent differentiation of rat ovarian theca cells. Biol. Reprod. 64:451.
Steinmetz et al. (2000) Effects of growth hormone-releasing hormone-related peptide on stem cell factor expression in cultured rat Sertoli cells. Endocrine. 12:323.
Yan et al. (2000) Involvement of Bcl-2 family proteins in germ cell apoptosis during testicular development in the rat and pro-survival effect of stem cell factor on germ cells in vitro. Mol. Cell. Endocrinol. 165:115.
Ferjan et al. (2000) Differential effect of interleukin-3, stem cell factor and nerve growth factor on histamine and serotonin release from rat mast cells. Inflamm. Res. 49:15.