Stem Cell Factor
mouse, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 18.3 kDa
Accession number: P20826
Accession number: P20826
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilized (SCF was lyophilized from 10 mM sodium phosphate buffer pH 6.5)
Solubility: It is recommended to reconstitute the lyophilized SCF in sterile bidest H2O not less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: The ED50 as determined by the dose-dependant stimulation of Human TF-1 cell line is < 10 ng/ml, corresponding to a Specific Activity of 1x105 IU/mg.
Stem Cell Factor (SCF) plays an important role in hematopoiesis and the survival, proliferation, and differentiation of mast cells, melanocytes, and germ cells. SCF mediates its biological effects by binding to and activating a receptor tyrosine kinase designated c-kit or SCF receptor (SCFR). SCF is also known as mast cell growth factor (MCGF), steel (Sl) factor (SLF), or kit ligand (KL). Recombinant mouse SCF produced in E. coli is a single, non-glycosylated polypeptide chain containing 164 amino acids and having a molecular mass of 18.3 kDa. The SCF is purified by proprietary chromatographic techniques.
Amino Acid Sequence: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
Ferret-Bernard et al. (2004) In vitro induction of inhibitory macrophage differentiation by granulocyte-macrophage colonystimulating factor, stem cell factor and interferon-gamma from lineage phenotypes-negative c-kit-positive murine hematopoietic progenitor cells. Immunol. Lett. 91:221.
Sykes et al. (2004) E2a/Pbx1 induces the rapid proliferation of stem cell factor-dependent murine pro-T cells that cause acute T-lymphoid or myeloid leukemias in mice. Mol. Cell. Biol. 24:1256.
Hartung et al. (2003) Enhanced antileukemic activity of allogeneic peripheral blood progenitor cell transplants following donor treatment with the combination of granulocyte colony-stimulating factor (GCSF) and stem cell factor (SCF) in a murine transplantation model. Bone Marrow Transplant. 32:49.
Ohmura et al. (2003) Increment of murine spermatogonial cell number by gonadotropin-releasing hormone analogue is independent of stem cell factor c-kit signal. Biol. Reprod. 68:2304.
Simpson et al. (2003) Stem cell factor attenuates liver damage in a murine model of acetaminophen-induced hepatic injury. Lab. Invest. 83:199.
Metcalf et al. (2002) Stem cell factor can stimulate the formation of eosinophils by two types of murine eosinophil progenitor cells. Stem Cells. 20:460.