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IL-11

Interleukin-11, adipogenesis inhibitory factor

human, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-471 10 μg 253,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 19.3 kDa

Accession number: P20809

Accession number: P20809

Purity: > 95 % (SDS-PAGE, RP-HPLC, FPLC)

Form: lyophilized

Solubility: It is recommended to reconstitute the lyophilized IL-11 in bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA)

Activity: ED50: < 10 ng/ml, corresponding to a specific activity 2 x 104 IU/mg, determined by the dosedependent proliferation of murine 7TD1 cells.

Description:
Interleukin-11 (IL-11) was initially cloned as a mediator of plasmacytoma cell proliferation and was later found to exhibit a wide variety of biological effects in neural cells as well as in the hematopoietic and the immune system. IL-11 inhibits the secretion of tumor necrosis factor (TNF), interleukin-12 (IL-12), interleukin-1β (IL-1β), and nitric oxide from activated macrophages as well as interferon γ and IL-12 from activated T cells. Recombinant human IL-11 produced in E. coli is a single, non-glycosylated polypeptide chain containing 179 amino acids and having a molecular mass of 19.3 kDa. IL-11 is purified by proprietary chromatographic techniques.

Selected References:
Kiessling et al. (2004) Functional expression of the interleukin-11 receptor alpha-chain and evidence of antiapoptotic effects in human colonic epithelial cells. J. Biol. Chem. 279:10304.
Hao et al. (2004) Effects of recombinant human interleukin 11 on thrombocytopenia and neutropenia in irradiated rhesus monkeys. Radiat. Res. 162:157.
Cotreau et al. (2004) A multiple-dose, safety, tolerability, pharmacokinetics and pharmacodynamic study of oral recombinant human interleukin-11 (oprelvekin). Biopharm. Drug. Dispos. 25:291.
Garzon-Rodriguez et al. (2004) Optimizing storage stability of lyophilized recombinant human interleukin-11 with disaccharide/hydroxyethyl starch mixtures. J. Pharm. Sci. 93:684.
Venkova et al. (2004) Oral treatment with recombinant human interleukin-11 improves mucosal transport in the colon of human leukocyte antigen-B27 transgenic rats. J. Pharmacol. Exp. Ther. 308:206.
Harmegnies et al. (2003) Characterization of a potent human interleukin-11 agonist. Biochem. J. 375:23.