Interleukin 3, mast cell growth factor
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 15 kDa
Accession number: P08700
Accession number: P08700
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Solubility: It is recommended to reconstitute the lyophilized IL-3 in bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: ED50: < 0.1 ng/ml, corresponding to a specific activity of 2 x 106 IU/mg, determined by the dosedependent stimulation of TF-1 cells.
Interleukin-3 (IL-3) is a cytokine produced by activated T-cells and mast cells that has been shown to stimulate renewal of pluripotent hematopoietic stem cells and to be a potent regulator of many hematopoietic cell lineages. It is involved in stimulating inducible hematopoiesis in response to parasite infections, and it has also been implicated in the pathogenesis of several chronic inflammatory diseases, including asthma, and neurodegenerative disorders, such as multiple sclerosis. Recombinant human IL-3 produced in E. coli is a single, non-glycosylated form of human IL-3 polypeptide chain containing 133 amino acids and having a molecular mass of 15 kDa. IL-3 is purified by proprietary chromatographic techniques.
Endotoxin: Less than 0.1 ng/μg (IEU/μg) of IL-3.
Murphy et al. (2004) Interleukin-3 binding to the murine betaIL-3 and human betac receptors involves functional epitopes formed by domains 1 and 4 of different protein chains. J. Biol. Chem. 279:26500.
Cohen et al. (2004) Toxicology and pharmacokinetics of DT388IL3, a fusion toxin consisting of a truncated diphtheria toxin (DT388) linked to human interleukin 3 (IL3), in cynomolgus monkeys. Leuk. Lymphoma. 45:1647.
Ding et al. (2003) Molecular cloning, expression, purification, and characterization of soluble full-length, human interleukin-3 with a baculovirus-insect cell expression system. Protein Expr. Purif. 31:34.
Biesma et al. (2002) Recombinant human interleukin-3 administered concomitantly with chemotherapy in patients with relapsed small cell lung cancer. J. Exp. Ther. Oncol. 2:47.
Zanetta et al. (2002) Evidence for a lectin activity for human interleukin 3 and modeling of its carbohydrate recognition domain. J. Biol. Chem. 277:38764.