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FLT3 Ligand

Fms-like Tyrosine Kinase 3 Ligand

human, recombinant, E. coli

Product Cat. No. Amount Price (EUR) Buy / Note
FLT3 Ligand PR-419 10 μg 253,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 18 kDa

Accession number: P49771

Accession number: P49771

Purity: > 95 % (SDS-PAGE, RP-HPLC)

Form: lyophilized

Solubility: It is recommended to reconstitute the lyophilized FLT3 in sterile bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Activity: EC50: < 1 ng/ml, corresponding to a specific activity of 1 x 106 Units/mg, calculated by the dosedependant stimulation of the proliferation of human AML5 cells.

Description:
FLT-3 Ligand binds to Fms-Like Tyrosine Kinase-3 (FLT-3). Human FLT3 ligand also stimulates the proliferation of cells expressing murine FLT-3 receptors. The effects of FLT-3 ligand are synergized by coexpression of G-CSF (cat.# PR-423), GM-CSF (cat.# PR-436), M-CSF (cat.# PR-491), IL-3 (cat.# PR-462), and SCF (cat.# PR-682). FLT-3 Ligand promotes long-term expansion and differentiation of human pro-B-cells in the presence of IL-7 (cat.# PR-468) or in combination of IL-7 and IL-3. Recombinant human FLT3 Ligand produced in E. coli is non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17.6 kDa. Recombinant FLT3 Ligand is purified by proprietary chromatographic techniques.

Endotoxin: Less than 0.1 ng/μg (IEU/μg) of FLT3.

Selected References:
Edwan et al. (2005) Treatment with Flt3 ligand plasmid reverses allergic airway inflammation in ovalbuminsensitized and -challenged mice. Int. Immunopharmacol. 5:345.
Chen et al. (2005) FLT3 ligand administration after hematopoietic cell transplantation increases circulating dendritic cell precursors that can be activated by CpG oligodeoxynucleotides to enhance T-cell and natural killer cell function. Biol. Blood Marrow Transplant. 11:23.
Fukuda et al. (2005) Flt3-ligand and the Flt3 receptor regulate hematopoietic cell migration by modulating the SDF- 1{alpha} (CXCL12)/CXCR4 axis. Blood 105:3117.
Coates et al. (2004) CCR and CC chemokine expression in relation to Flt3 ligand-induced renal dendritic cell mobilization. Kidney Int. 66:1907.
Swanson et al. (2004) Flt3-ligand, IL-4, GM-CSF, and adherencemediated isolation of murine lung dendritic cells: assessment of isolation technique on phenotype and function. J. Immunol. 173:4875.
Shackleton et al. (2004) The impact of imiquimod, a Toll-like receptor-7 ligand (TLR7L), on the immunogenicity of melanoma peptide vaccination with adjuvant Flt3 ligand. Cancer Immun. 4:9.