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MIA

Melanoma Inhibitory Activity Protein

human, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-492 20 μg 253,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 12 kDa

Accession number: Q16674

Accession number: Q16674

Purity: > 95 % (SDS-PAGE, RP-HPLC)

Form: lyophilized (MIA protein was lyophilized from a solution containing 20 mM Potassium phosphate pH 7.0 and 150 mM KCl)

Solubility: It is recommended to reconstitute the lyophilized MIA in sterile bidest H2O not less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Description:
MIA (Melanoma Inhibitory Activity Protein) acts as a potent tumor cell growth inhibitor for malignant melanoma cells and some other neuroectodermal tumors, including gliomas, in an autocrine fashion. In a study of human melanoma cell lines with different metastatic capacity MIA mRNA expression appeared to be inversely correlated with pigmentation. MIA has been shown to represent a very sensitive and specific serum marker for systemic malignant melanoma that might be useful for staging of primary melanomas, detection of progression from localized to metastatic disease during follow-up, and monitoring therapy of advanced melanomas. Recombinant human MIA produced in E. coli is a single, non-glycosylated, polypeptide chain consisting of 108 amino acids having a total molecular mass of 12.237 kDa. MIA was identified as an inhibitor of in vitro growth of malignant melanoma cells. The protein contains a SH3 domain. Human Melanoma Inhibitory protein is purified by proprietary chromatographic techniques.

Endotoxin: Less than 0.1 ng/μg (IEU/μg) of MIA.

Selected References:
Lougheed et al. (2002) Solution structure and dynamics of melanoma inhibitory activity protein. J. Biomol. NMR. 22:211.
Lougheed et al. (2001) Structure of melanoma inhibitory activity protein, a member of a recently identified family of secreted proteins. Proc. Natl. Acad. Sci. USA 98:5515.
Dreau et al. (1999) Melanoma-inhibitory activity protein concentrations in blood of melanoma patients treated with immunotherapy. Oncol. Res. 11:55.