CD40L - soluble

CD40 Ligand/CD154/TRAP TNF-related Activation Protein

human, recombinant, E. coli

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CD40L - soluble PR-680 50 μg 253,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 16.3 kDa

Accession number: P29965

Accession number: P29965

Purity: > 95 % (SDS-PAGE, RP-HPLC)

Form: lyophilized (with 10 mM sodium phosphate pH 7.5)

Solubility: It is recommended to reconstitute the lyophilized CD40L in sterile bidest H2O not less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Activity: ED50: 5-10 ng/ml, determined by the dosedependent stimulation of IL-12 and IL-8 induction by PBM (Peripheral Mononuclear) cells.

Description:
Recombinant Human CD40L produced in E. coli is a non-glycosylated polypeptide chain containing 149 amino acids and having a molecular mass of 16.3 kDa. CD40L or CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. CD40L stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40L has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also costimulates proliferation of activated T-cells and this is accompanied by the production of IFN-γ, TNF-α, and IL-2. Recombinant CD40L is purified by proprietary chromatographic techniques.

Amino Acid Sequence: The sequence of the first five N-terminal amino acids was found to be Met-Gln-Lys-Gly-Asp.

Selected References:
Cognasse et al. (2005) Differential downstream effects of CD40 ligation mediated by membrane or soluble CD40L and agonistic Ab: a study on purified human B cells. Int. J. Immunopathol. Pharmacol. 18:65.
Guiducci et al. (2005) CD40/CD40L interaction regulates CD4 (+)CD25 (+) T reg homeostasis through dendritic cell-produced IL-2. Eur. J. Immunol. 35:557.
Novo et al. (2005) Soluble CD40L and Cardiovascular Risk in Asymptomatic Low-Grade Carotid Stenosis. Stroke. 36:673.
Rayat et al. (2005) Indefinite Survival of Neonatal Porcine Islet Xenografts by Simultaneous Targeting of LFA-1 and CD154 or CD45RB. Diabetes. 54:443.
Farahani et al. (2005) Autocrine VEGF mediates the antiapoptotic effect of CD154 on CLL cells. Leukemia. 19:524.