Interleukin-6, B-cell hybridoma growth factor
murine, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 22 kDa
Accession number: P08505
Accession number: P08505
Purity: > 90 % (SDS-PAGE)
Form: lyophilized (IL-6 is lyophilized from a 1 mg/ml solution containing 10 mM NaAcetate pH 6.0)
Solubility: It is recommended to reconstitute the lyophilized Interleukin 6 in 20 mM HCl to 0.1-1.0 mg/ml to regain full activity and can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: ED50: < 0.02 ng/ml, corresponding to a specific activity 5 x 107 IU/mg, determined by the dosedependent stimulation of murine 7TD1 cells.
IL-6 is a variably glycosylated protein produced by many different cells, but the main sources in vivo are stimulated monocytes/macrophages, fibroblasts, and vascular endothelial cells, indicative of its role in the modulation of the immune system. Other cells known to express IL-6 include keratinocytes, osteoblasts, T-cells, B-cells, neutrophils, eosinophils, mast cells, smooth muscle cells, and skeletal muscle cells. Typical stimuli for IL-6 production are IL-1, TNF-α, and bacterial endotoxin. Recombinant murine IL-6 produced in E. coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21.7 kDa. IL-6 is purified by proprietary chromatographic techniques.
Endotoxin: Less than 0.1 ng/μg (IEU/μg) of IL-6.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser.
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Meads et al. (2004) Kaposi'ssarcomaassociated herpesvirus-encoded viral interleukin-6 is secreted and modified differently than human interleukin-6: evidence for a unique autocrine signaling mechanism. J. Biol. Chem. 279:51793.
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Matthews et al. (2003) Cellular cholesterol depletion triggers shedding of the human interleukin-6 receptor by ADAM10 and ADAM17 (TACE). J. Biol. Chem. 278:38829.
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Varghese et al. (2002) Structure of the extracellular domains of the human interleukin-6 receptor alpha -chain. Proc. Natl. Acad. Sci. USA. 99:15959.
Song et al. (2002) Human interleukin-6 induces human herpesvirus- 8 replication in a body cavity-based lymphoma cell line. J. Med. Virol. 68:404.