Interleukin-5, B-cell differentiation Factor
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 26.5 kDa
Accession number: P05113
Accession number: P05113
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Solubility: It is recommended to reconstitute the lyophilized IL-5 in bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: ED50: < 0.15 ng/ml, corresponding to a specific activity of 1.3 x 106 IU/mg, determined by the dose-dependent stimulation of the proliferation of TF-1 cells.
In humans, interleukin-5 (IL-5) acts only on eosinophils and basophils, in which it causes maturation, growth, activation, and survival. Several allergic diseases, such as nasal rhinitis, nasal polyps, asthma, idiopathic eosinophilic syndromes, and atopic dermatitis, have prominent inflammatory components that are characterized by pronounced eosinophilic infiltration. Recombinant human IL-5 produced in E. coli is a dimeric, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 26.522 kDa. IL-5 is purified by proprietary chromatographic techniques.
Endotoxin: Less than 0.1 ng/μg (IEU/μg) of IL-5.
DiGiacomo et al. (2004) Development and validation of a kinetic assay for analysis of anti-human interleukin-5 monoclonal antibody (SCH 55700) and human interleukin-5 interactions using surface plasmon resonance. Anal. Biochem. 327:165.
Ishino et al. (2004) Kinetic interaction analysis of human interleukin 5 receptor alpha mutants reveals a unique binding topology and charge distribution for cytokine recognition. J. Biol. Chem. 279:9547.
Feng et al. (2002) Sequence-specific NMR resonance assignments for human interleukin-5. J. Biomol. NMR. 23:329.
Schwenger et al. (2002) Specific activation of human interleukin-5 depends on de novo synthesis of an AP-1 complex. J. Biol. Chem. 277:47022.
Greenfeder et al. (2001) Th2 cytokines and asthma - The role of interleukin-5 in allergic eosinophilic disease. Respir. Res. 2:71.
Schwenger et al. (2001) GATA-3 has dual regulatory functions in human interleukin-5 transcription. J. Biol. Chem. 276:48502.
Karras et al. (2000) Antisense inhibition of membrane-bound human interleukin-5 receptor-alpha chain does not affect soluble receptor expression and induces apoptosis in TF-1 cells. Antisense Nucleic Acid Drug Dev. 10:347.