B Lymphocyte Stimulator/ B cell-activating factor belonging to the TNF family
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 17 kDa
Accession number: Q9Y275
Accession number: Q9Y275
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilized (Each mg of Human BAFF contains 1.6 mg mannitol and 1.2 mg HSA)
Solubility: It is recommended to reconstitute the lyophilized BAFF in sterile bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
Activity: ED50: < 10 ng/ml corresponding to a Specific Activity of 105 IU/mg.This was determined by the dosedependent stimulation of IL-8 production by human Peripheral Blood Mononuclear Cells (PBMCs).
The TNF-related ligand, B cell-activating factor belonging to the TNF family (BAFF), is necessary for normal B cell development and survival, and specifically binds the receptors transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI), B cell maturation Antigen (BCMA), and BAFF-R. Using specific mAbs, BAFF-R was found to be the predominant receptor expressed on peripheral B cells, in both humans and mice. BAFF expression on human monocytes could be upregulated by IFNγ. BAFF produced in E. coli is a single, non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of 17 kDa. Recombinant BAFF is purified by proprietary chromatographic techniques.
Amino Acid Sequence: MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL
Moore et al. (1999) BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator. Science 285:260.
Yamada et al. (2005) B Lymphocyte Stimulator activates p38 MAPK in Human Ig Class Switch Recombination. Am. J. Respir. Cell Mol. Biol. Jan 24, [Epub ahead of print].
Morrison et al. (2005) An atypical TRAF-binding motif of BAFF receptor mediates induction of the noncanonical NF-kB signaling pathway. J. Biol. Chem. 280:10018.
Krumbholz et al. (2005) BAFF is produced by astrocytes and upregulated in multiple sclerosis lesions and primary central nervous system lymphoma. J. Exp. Med. 201:195.
Laird et al. (2005) Optimization of BLyS production and purification from Escherichia coli. Protein Expr. Purif. 39:237.
Gottenberg et al. (2005) Serum B lymphocyte stimulator and beta2- microglobulin correlation with autoantibody secretion and systemic involvement in primary Sjogren's syndrome. Ann. Rheum. Dis. 64:114.