ovine (sheep), recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 23 kDa
Accession number: P01240
Accession number: P01240
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilised (Prolactin was lyophilised from a solution with 0.0045 mM NaHCO3)
Solubility: It is recommended to reconstitute the lyophilised Prolactin in sterile bidest H2O not less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: ED50: < 0.065 ng/ml, determined by the dosedependent stimulation of the proliferation of rat lymphoma, Nb2-11.
Prolactin is a lactogenic hormone secreted by the adenohypophysis. Besides its major action on lactation, in some species prolactin exerts effects on reproduction, maternal behavior, fat metabolism, immunomodulation, and osmoregulation. Prolactin has been shown also to have cytokine-like and important immunoregulatory activities. It contributes to the development of lymphoid tissues and the maintenance of physiological immune function and also modulates a variety of T-cell immune responses. Prolactin has been reported to activate cellular proliferation in nonreproductive tissue, such as liver, spleen, and thymus. It induces significant proliferation in aortic smooth muscle cells and also enhances proliferation of these cells induced by PDGF. Prolactin also appears to be directly mitogenic for pancreatic beta cells. Prolactin is also mitogenic for cultured astrocytes. Recombinant Ovine Prolactin produced in E. coli is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 23 kDa. Recombinant ovine Prolactin is purified by proprietary chromatographic techniques.
Endotoxin: Less than 0.1 ng/μg (IEU/μg) of Prolactin.
Leibovich et al. (2001) Large-scale preparation of recombinant ovine prolactin and determination of its in vitro and in vivo activity. ProteinExpr. Purif. 22:489.
Fernandez et al. (2000) Ovine placental lactogen and ovine prolactin: partial proteolysis and conformational stability. Int. J. Biochem. Cell. Biol. 32:597.
Bignon et al. (1999) In vitro expression of long and short ovine prolactin receptors: activation of Jak2/STAT5 pathway is not sufficient to account for prolactin signal transduction to the ovine beta-lactoglobulin gene promoter. J. Mol. Endocrinol. 23:125.
Vincent et al. (1997) Rapid communication: a restriction fragment length polymorphism in the ovine Prolactin gene. J. Anim. Sci. 75:1686.
Luquita et al. (1996) Ovine prolactin increases hepatic UDPglucuronosyltransferase activity in ovariectomized rats. J. Pharmacol. Exp. Ther. 278:921.
Muccioli et al. (1994) Modulation of prolactin receptors in the rat hypothalamus in response to changes in serum concentration of endogenous prolactin or to ovine prolactin administration. Brain Res. 663:244.