» Sign in / Register


Methionine-R-Sulfoxide Reductase B3, EC1.8.4.6

human, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-133 100 μg 207,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 22.5 kDa

Accession number: AAH40053

Accession number: AAH40053

Purity: > 90 % (SDS-PAGE)

Form: liquid (Supplied in 0.1 M Tris-HCl pH 7.5 and 50% glycerol)

Activity: 1 nmol of hMSRB3 will reduce 2 nmol peptide-bound Met-S-sulfoxide in 1 min at 37°C.

MSRB3 is His-tagged at the N-terminal. MSRB3 (Methionine-R-sulfoxide reductase, EC1.8.4.6, accession number AAH40053) is one of three different mammalian MSRB enzymes. It carries out the reduction of methionine-R-sulfoxide to methionine. Human studies have shown the highest levels of expression in smooth muscle tissues. Its proposed function is the repair of oxidative damage to proteins to restore biological activity.

Activity assay: MetO-containing peptides are reduced by the enzyme in the presence of DTT.

Selected References:
Hansel et al. (2005) Heterogeneity and function of mammalian MSRs: enzymes for repair, protection and regulation. Biochim. Biophys. Acta 1703:239.
Marchetti et al. (2005) Methionine sulfoxide reductases B1, B2, and B3 are present in the human lens and confer oxidative stress resistance to lens cells. Invest. Ophthalmol. Vis. Sci 46:2107.
Kim et al. (2004) Methionine sulfoxide reduction in mammals: characterization of methionine-R-sulfoxide reductases. Mol. Biol. Cell 15:1055.
Hansel et al. (2003) A second human methionine sulfoxide reductase (hMSRB2) reducing methionine-R-sulfoxide displays a tissue expression pattern distinct from hMSRB1. Redox Report 8:384.