MSRBHis

Methionine-R-Sulfoxide Reductase B, EC1.8.4.6

E. coli, recombinant, E. coli

Product Cat. No. Amount Price (EUR) Buy / Note
MSRBHis PR-132 100 μg 207,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 16.8 kDa

Accession number: NP_416292

Accession number: NP_416292

Purity: > 90 % (SDS-PAGE)

Form: liquid (Supplied in 100 mM Tris-HCl pH 7.4 and 33% glycerol)

pH: 7.4

Activity: 1 nmol of E. coli MSRB will reduce 0.6 nmol peptide-bound Met-R-sulfoxide in 1 min at 37°C.

Description:
MSRB is His-tagged at the N-terminal. Like most bacteria, Escherichia coli expresses a single MSRB (Methionine-R-sulfoxide reductase, EC1.8.4.6; accession number NP416292) that carries out the reduction of methionine-R-sulfoxide to methionine. The E. coli enzyme - like the two human enzymes offered by Jena Bioscience - belong to a group of MsrBs, that possess a metal binding site composed of two CXXC motifs. The bound metal (zinc or iron) may stabilize the conformation of the enzymes.

Activity assay: MetO-containing peptides are reduced by the enzyme in the presence of DTT.

Selected References:
Ezraty et al. (2005) Methionine sulfoxide reductases in prokaryotes. Biochim. Biophys. Acta 1703:221.
Olry et al. (2005) Insights into the role of the metal binding site in methionine-R-sulfoxide reductases B. Protein Sci. 14:2828.
Etienne et al. (2003) A methionine sulfoxide reductase in Escherichia coli that reduces the R-enantiomer of methionine sulfoxide. Biochem. Biophys. Res. Commun. 300:378.
Grimaud et al. (2001) Repair of oxidized proteins. Identification of a new methionine sulfoxide reductase. J. Biol. Chem. 276:48915.
Schallreuter et al. (2007) Methionine Sulfoxide Reductases A and B Are Deactivated by Hydrogen Peroxide (H2O2) in the Epidermis of Patients with Vitiligo. Journal of Investigative Dermatology 128:808-815.