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Methionine Sulfoxide Reductase B2, EC1.8.4.6

human, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-131 200 μg 207,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 6 months

Molecular Weight: 17 kDa

Accession number: NP_036360

Accession number: NP_036360

Purity: > 90 % (SDS-PAGE)

Form: liquid (Supplied in 50 mM Tris-HCl pH 7.4 and 50% glycerol)

Activity: 1 nmol of hMSRB2 will reduce 3 nmol peptide-bound Met-R-sulfoxide in 1 min at 37°C.

MSRB2 is His-tagged at the N-terminal. MSRB2 (Methionine sulfoxide reductase, EC1.8.4.6) is ubiquitous and highly conserved. It carries out the enzymatic reduction of methionine-R-sulfoxide (MetRO) to methionine in thioredoxin-dependent manner. Human and animal studies have shown the highest levels of expression in skeletal muscle and heart. Its proposed function is the repair of oxidative damage to proteins to restore biological activity. The protein is located in mitochondria.

Activity assay: MetRO-containing peptides and free MetRO are reduced by the enzyme in the presence of DTT.

Selected References:
Binger et al. (2010) Methionine-Oxidized Amyloid Fibrils Are Poor Substrates for Human Methionine Sulfoxide Reductases A and B2. Biochemistry 49:2981.
Hansel et al. (2005) Heterogeneity and function of mammalian MSRs: enzymes for repair, protection and regulation. Biochim. Biophys. Acta 1703:239.
Kim et al. (2004) Methionine sulfoxide reduction in mammals: characterization of methionine-R-sulfoxide reductases. Mol. Biol. Cell 15:1055.
Jung et al. (2002) Activity, tis sue distribution and site-directed mutagenesis of a human peptide methionine sulfoxide reductase of type B: hCBS1. FEBS Lett. 527:91.