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Methionine Sulfoxide Reductase A, EC1.8.4.6

human, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-130 100 μg 207,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 25.9 kDa

Accession number: NP_036463

Accession number: NP_036463

Purity: > 90 % (SDS-PAGE)

Form: liquid (Supplied in 0.1 M Tris-HCl pH 7.4 and 30% glycerol)

Activity: 1 nmol of hMSRA will reduce 7 nmol peptide-bound Met-S-sulfoxide in 1 min at 37°C.

MSRA is His-tagged at the N-terminal. MSRA (Methionine sulfoxide reductase, EC1.8.4.6) is ubiquitous and highly conserved. It carries out the enzymatic reduction of methionine-S-sulfoxide (MetSO) to methionine in thioredoxin-dependent manner. Human and animal studies have shown the highest levels of expression in kidney and nervous tissue. Its proposed function is the repair of oxidative damage to proteins to restore biological activity. The two known splice variants of the mammalian protein are located in mitochondria and the cytosol, respectively.

Selected References:
Binger et al. (2010) Methionine-Oxidized Amyloid Fibrils Are Poor Substrates for Human Methionine Sulfoxide Reductases A and B2. Biochemistry 49:2981.
Hansel et al. (2005) Heterogeneity and function of mammalian MSRs: enzymes for repair, protection and regulation. Biochim. Biophys. Acta 1703:239.
Kuschel et al. (1999) Molecular cloning and functional expression of a human peptide methionine sulfoxide reductase (hMsrA) FEBS Lett. 456:17.
Schallreuter et al. (2007) Methionine Sulfoxide Reductases A and B Are Deactivated by Hydrogen Peroxide (H2O2) in the Epidermis of Patients with Vitiligo. Journal of Investigative Dermatology 128:808.
Ghesquie` re et al. (2011) Redox proteomics of protein-bound methionine oxidation. Molecular and Celluar Proteomics 10 (5).