» Sign in

PrPc (globular domain/residues 90-231)His

Prion Protein, cellular form

human, recombinant, E. coli

Product Cat. No. Amount Price (EUR) Buy / Note
PrPc (globular domain/residues 90-231)His PR-905 50 μg 258,75 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 18.5 kDa

Accession number: NM_000311 / P04156

Accession number: NM_000311 / P04156

Purity: > 90 % (SDS-PAGE)

Form: lyophilized (Dialyzed before against 0.1 μM HCl)

Description:
N-terminal His-tagged prion protein globular domain (amino acid residues 90-231). The protein may be reconstituted in detergent-containing buffers e.g. TX-100 (0.5%) or under mild denaturing conditions (e.g. 1.5 M guanidine-HCl or urea). The His-tagged PrPc is an ideal positive control in immunochemical detection of prions. It may be used directly for the production of antibodies in animals.

Selected References:
Prusiner (1998) Prions. Proc. Natl. Acad. Sci. USA 95:13363.
Pan et al. (1993) Conversion of α-helices into β-sheets features in the formation of the scrapie prion proteins. Proc. Natl. Acad. Sci. USA 90:10962.
Lee et al. (1998) Complete genomic sequence and analysis of the prion protein gene region from three mammalian species. Genome Res. 8:1022.
Bergstrom et al. (2005) Amidation and Structure Relaxation Abolish the Neurotoxicity of the Prion Peptide PrP106-126 in vivo and in vitro. J. Biol. Chem. 280:23114.