γ-Aminophenyl-ATP-Agarose (no spacer)
Aminophenyl-Adenosine triphospate (AP-ATP) immobilized on Agarose
For research use only!
Shipping: shipped at 4 °C
Storage Conditions: store at 4 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Suitable for purification of ATP-binding proteins.
Degree of substitution: 25 μmol - 27 μmol AP-ATP/ml gel
Storage buffer: 20 % Ethanol
Important note: The use of this reagent for the purification of protein kinases is subject to United States Patent No. 5, 536, 822. Such use is prohibited without a license from Serenex, Inc. Information on obtaining a license is available at firstname.lastname@example.org.
Mlakar et al. (2006) Citrate Inhibition-Resistant Form of 6-Phosphofructo-1-Kinase from Aspergillus niger. Applied and Environmental Microbiology 72 (7):4515.
Drewes et al. (1995) Microtubule-associated Protein/Microtubule Affinity-regulating Kinase (p110mark). J. Biol. Chem. 270:7679.
Haystead et al. (1993) γ-phosphate linked ATPSepharose for the affinity purification of protein-kinases - rapid purification to homogeneity of sceletal-muscle mitogenactivated protein-kinase. Eur. J. Biochem. 214:459.
Usenik et al. (2010) Evolution of allosteric citrate binding sites on 6-phosphofructo-1-kinase. PLoS One. 5 (11):e15447.