Suitable for small-scale & automated purification of polyhistidine (His)-tagged proteins.
Nickel-NTA Magnetic Agarose Beads consist of a ferrimagnetic core that is coated with 6 % cross-linked Agarose coupled to Ni2+-ions via a Nitrilotriacetic acid (NTA)-ligand.
The tetradentate NTA-linker generally results in tighter protein binding and reduced metal leaching, compared to the tridentate Iminodiacetic (IDA)-version.
Ni-NTA Magnetic Agarose characteristics | |
|---|---|
| Matrix | Ferrimagnetic beads coated with 6 % cross-linked Ni-NTA Agarose |
| Ligand | Nickel (Ni2+) via an Nitrilotriacetic acid (NTA)-linker |
| Ligand density | > 12 µmol Ni2+/ml beads |
| Medium particle diameter | 30 µm (highly homogenous) |
| Protein Binding capacity* | 70 mg/ml beads |
| Recommended pH | between 3–12 |
| Chemical stability | Stable to all solutions commonly used during the purification procedure (refer to the datasheet) |
