Suitable for small-scale & automated purification of polyhistidine (His)-tagged proteins.
Nickel-NTA Magnetic Agarose Beads consist of a ferrimagnetic core that is coated with 6 % cross-linked Agarose coupled to Ni2+-ions via a Nitrilotriacetic acid (NTA)-ligand.
The tetradentate NTA-linker generally results in tighter protein binding and reduced metal leaching, compared to the tridentate Iminodiacetic (IDA)-version.
Ni-NTA Magnetic Agarose characteristics
|Matrix||Ferrimagnetic beads coated with 6 % cross-linked Ni-NTA Agarose|
|Ligand||Nickel (Ni2+) via an Nitrilotriacetic acid (NTA)-linker|
|Ligand density||> 12 µmol Ni2+/ml beads|
|Medium particle diameter||30 µm (highly homogenous)|
|Protein Binding capacity*||70 mg/ml beads|
|Recommended pH||between 3–12|
|Chemical stability||Stable to all solutions commonly used during the purification procedure (refer to the datasheet)|