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Nickel NTA Magnetic Agarose Beads

Suitable for small-scale & automated purification of polyhistidine (His)-tagged proteins.

Nickel-NTA Magnetic Agarose Beads consist of a ferrimagnetic core that is coated with 6 % cross-linked Agarose coupled to Ni2+-ions via a Nitrilotriacetic acid (NTA)-ligand.

The tetradentate NTA-linker generally results in tighter protein binding and reduced metal leaching, compared to the tridentate Iminodiacetic (IDA)-version.

Ni-NTA Magnetic Agarose characteristics

MatrixFerrimagnetic beads coated with 6 % cross-linked Ni-NTA Agarose
LigandNickel (Ni2+) via an Nitrilotriacetic acid (NTA)-linker
Ligand density> 12 µmol Ni2+/ml beads
Medium particle diameter30 µm (highly homogenous)
Protein Binding capacity*70 mg/ml beads
Recommended pHbetween 3–12
Chemical stabilityStable to all solutions commonly used during the purification procedure (refer to the datasheet)
* Tested with His - GFP (~32 kDa); Binding capacity may vary depending on amino acid sequence, molecular weight and His-tag location.