Nickel NTA Magnetic Agarose Beads

Suitable for small-scale & automated purification of polyhistidine (His)-tagged proteins.

Nickel-NTA Magnetic Agarose Beads consist of a ferrimagnetic core that is coated with 6 % cross-linked Agarose coupled to Ni²⁺-ions via a Nitrilotriacetic acid (NTA)-ligand.

The tetradentate NTA-linker generally results in tighter protein binding and reduced metal leaching, compared to the tridentate Iminodiacetic (IDA)-version.

Ni-NTA Magnetic Agarose characteristics
Matrix Ferrimagnetic beads coated with 6 % cross-linked Ni-NTA Agarose
Ligand Nickel (Ni²⁺) via an Nitrilotriacetic acid (NTA)-linker
Ligand density > 12 µmol Ni²⁺/ml beads
Medium particle diameter 30 µm (highly homogenous)
Protein Binding capacity* 70 mg/ml beads
Recommended pH between 3–12
Chemical stability Stable to all solutions commonly used during the purification procedure (refer to the datasheet)

* Tested with His - GFP (~32 kDa); Binding capacity may vary depending on amino acid sequence, molecular weight and His-tag location.

Ni-NTA Magnetic Agarose characteristics
Matrix	Ferrimagnetic beads coated with 6 % cross-linked Ni-NTA Agarose
Ligand	Nickel (Ni²⁺) via an Nitrilotriacetic acid (NTA)-linker
Ligand density	> 12 µmol Ni²⁺/ml beads
Medium particle diameter	30 µm (highly homogenous)
Protein Binding capacity*	70 mg/ml beads
Recommended pH	between 3–12
Chemical stability	Stable to all solutions commonly used during the purification procedure (refer to the datasheet)

Products & Ordering

Ni-NTA Magnetic Agarose Beads AC-604

Nickel NTA Magnetic Agarose Beads

Products & Ordering

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