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Cobalt NTA Magnetic Agarose Beads

Suitable for small-scale & automated purification of polyhistidine (His)-tagged proteins.

Cobalt-NTA Magnetic Agarose Beads consist of a ferrimagnetic core that is coated with 6 % cross-linked Agarose coupled to Co2+-ions via a Nitrilotriacetic acid (NTA)-ligand.

Compared to Ni2+ ions, Co2+-ions generally display a higher specificity towards histidines and are therefore an ideal option to reduce non-specific protein binding. The tetradentate NTA-linker generally results in tighter protein binding and reduced metal leaching, compared to the tridentate Iminodiacetic (IDA)-version.

Co-NTA Magnetic Agarose characteristics

MatrixFerrimagnetic beads coated with 6 % cross-linked Co-NTA Agarose
LigandCobalt (Co2+) via an Nitrilotriacetic acid (NTA)-linker
Ligand density> 13 µmol Co2+/ml beads
Medium particle diameter30 µm (highly homogenous)
Protein Binding capacity*30 mg protein per ml settled beads
Recommended pHbetween 3 - 12
Chemical stabilityStable to all solutions commonly used during the purification procedure (refer to the datasheet)
*Determined with purified His - GFP (~32 kDa) from cleared E. coli protein lysate and spectroscopic fluorescence measurement; Binding capacity may vary depending on amino acid sequence, molecular weight and His-tag location.