Suitable for small-scale & automated purification of polyhistidine (His)-tagged proteins.
Cobalt-NTA Magnetic Agarose Beads consist of a ferrimagnetic core that is coated with 6 % cross-linked Agarose coupled to Co2+-ions via a Nitrilotriacetic acid (NTA)-ligand.
Compared to Ni2+ ions, Co2+-ions generally display a higher specificity towards histidines and are therefore an ideal option to reduce non-specific protein binding. The tetradentate NTA-linker generally results in tighter protein binding and reduced metal leaching, compared to the tridentate Iminodiacetic (IDA)-version.
Co-NTA Magnetic Agarose characteristics
|Matrix||Ferrimagnetic beads coated with 6 % cross-linked Co-NTA Agarose|
|Ligand||Cobalt (Co2+) via an Nitrilotriacetic acid (NTA)-linker|
|Ligand density||> 13 µmol Co2+/ml beads|
|Medium particle diameter||30 µm (highly homogenous)|
|Protein Binding capacity*||30 mg protein per ml settled beads|
|Recommended pH||between 3 - 12|
|Chemical stability||Stable to all solutions commonly used during the purification procedure (refer to the datasheet)|