» Sign in / Register

Nickel IDA Magnetic Agarose Beads

Suitable for small-scale & automated purification of polyhistidine (His)-tagged proteins.

Nickel-IDA Magnetic Agarose Beads consist of a ferrimagnetic core that is coated with 6 % cross-linked Agarose coupled to Ni2+-ions via an Iminodiacetic acid (IDA)-linker.

Ni-IDA Magnetic Agarose characteristics

MatrixFerrimagnetic beads coated with 6 % cross-linked Ni-IDA Agarose
LigandNickel (Ni2+ via an Iminodiacetic acid (IDA)-linker
Ligand density> 15 µmol Ni2+/ml beads
Medium particle diameter30 µm (highly homogenous)
Protein Binding capacity*40 mg/ml beads
Recommended pHbetween 3 - 12
Chemical stabilityStable to all solutions commonly used during the purification procedure (refer to the datasheet)
* Tested with His - GFP (~32 kDa); Binding capacity may vary depending on amino acid sequence, molecular weight and His-tag location.