Suitable for small-scale & automated purification of polyhistidine (His)-tagged proteins.
Cobalt-IDA Magnetic Agarose Beads consist of a ferrimagnetic core that is coated with 6 % cross-linked Agarose coupled to Co2+-ions via an Iminodiacetic acid (IDA)-linker.
Compared to Ni2+ ions, IDA-immobilized Co2+-ions generally display a higher specificity towards histidines and are therefore an ideal option to reduce non-specific protein binding.
Co-IDA Magnetic Agarose characteristics
|Matrix||Ferrimagnetic beads coated with 6 % cross-linked Co-IDA Agarose|
|Ligand||Cobalt (Co2+ via an Iminodiacetic acid (IDA)-linker|
|Ligand density||> 15 µmol Co2+/ml beads|
|Medium particle diameter||30 µm (highly homogenous)|
|Protein Binding capacity*||40 mg/ml beads|
|Recommended pH||between 3 - 12|
|Chemical stability||Stable to all solutions commonly used during the purification procedure (refer to the datasheet)|