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Suitable for FPLC, gravity flow and batch purification
6 % Nickel-IDA Agarose is a superior, 6 % cross-linked immobilized metal affinity chromatography (IMAC) resin that uses nickel ions for purifying recombinant polyhistidine (His-) tagged proteins. Its unique properties facilitate the rapid and high-yielding one-step purification from crude lysates both under denaturing and non-denaturing conditions.
Nickel-IDA Agarose characteristics | |
|---|---|
| Matrix | 6 % cross-linked agarose |
| Linker | Iminodiacetic acid (IDA) |
| Bead size | 50 - 150 µm (cartridges: 20 - 50 µm) |
| Metal loading density | ~ 20 µmol Ni2+/ml gel |
| Protein binding capacity | 25 mg protein/ml gel (tested with Ribonucleotide reductase (RNR)) |
| Max. linear flow rate | ≥ 1000 cm/h |
| Maximum pressure | 3 bar (43.5 psi) |
| pH stability | 2-12 |
| Chemical stability | Stable to all solutions commonly used in gel filtration including 8 M urea and 6 M guanidine hydrochloride, autoclavable (120°C, 30') |
