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Nickel-IDA Agarose

Suitable for FPLC, gravity flow and batch purification

6 % Nickel-IDA Agarose is a superior, 6 % cross-linked immobilized metal affinity chromatography (IMAC) resin that uses nickel ions for purifying recombinant polyhistidine (His-) tagged proteins. Its unique properties facilitate the rapid and high-yielding one-step purification from crude lysates both under denaturing and non-denaturing conditions.

  • Excellent binding capacity based on high surface area to volume ratio
  • Fast flow rates
  • Notable mechanical and chemical stability
  • Reusable without any significant decrease in yield

Nickel-IDA Agarose characteristics

Matrix6 % cross-linked agarose
LinkerIminodiacetic acid (IDA)
Bead size50 - 150 µm (cartridges: 20 - 50 µm)
Metal loading density ~ 20 µmol Ni2+/ml gel
Protein binding capacity 25 mg protein/ml gel (tested with Ribonucleotide reductase (RNR))
Max. linear flow rate≥ 1000 cm/h
Maximum pressure3 bar (43.5 psi)
pH stability2-12
Chemical stabilityStable to all solutions commonly used in gel filtration including 8 M urea and 6 M guanidine hydrochloride,
autoclavable (120°C, 30')

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