Suitable for FPLC, gravity flow and batch purification
6 % Cobalt-IDA Agarose is a superior, 6 % cross-linked immobilized metal affinity chromatography (IMAC) resin that uses cobalt ions for purifying recombinant polyhistidine (His-) tagged proteins. Its unique properties facilitate the rapid and high-yielding one-step purification from crude lysates both under denaturing and non-denaturing conditions.
Cobalt-IDA Agarose characteristics
|Matrix||6 % cross-linked agarose|
|Linker||Iminodiacetic acid (IDA)|
|Bead size||50 - 150 µm|
|Metal loading density||~ 20 µmol Co2+/ml gel|
|Protein binding capacity||25 mg protein/ml gel (tested with Ribonucleotide reductase (RNR))|
|Max. linear flow rate||≥ 1000 cm/h|
|Maximum pressure||3 bar (43.5 psi)|
|Chemical stability||Stable to all solutions commonly used in gel filtration including 8 M urea and 6 M guanidine hydrochloride|
 Ueda et al. (2003) Current and prospective applications of metal ion–protein binding. Journal of Chromatography 988:1.