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Cobalt-IDA Agarose

Suitable for FPLC, gravity flow and batch purification

6 % Cobalt-IDA Agarose is a superior, 6 % cross-linked immobilized metal affinity chromatography (IMAC) resin that uses cobalt ions for purifying recombinant polyhistidine (His-) tagged proteins. Its unique properties facilitate the rapid and high-yielding one-step purification from crude lysates both under denaturing and non-denaturing conditions.

  • Excellent binding capacity based on high surface area to volume ratio
  • Fast flow rates
  • Notable mechanical and chemical stability
  • Reusable without any significant decrease in yield

Compared to Ni2+ ions, IDA-immobilized Co2+-ions generally display a higher specificity towards histidines and are therefore an ideal option to reduce non-specific protein binding[1].

Cobalt-IDA Agarose characteristics

Matrix6 % cross-linked agarose
LinkerIminodiacetic acid (IDA)
Bead size50 - 150 µm
Metal loading density ~ 20 µmol Co2+/ml gel
Protein binding capacity 25 mg protein/ml gel (tested with Ribonucleotide reductase (RNR))
Max. linear flow rate≥ 1000 cm/h
Maximum pressure3 bar (43.5 psi)
pH stability2-12
Chemical stabilityStable to all solutions commonly used in gel filtration including 8 M urea and 6 M guanidine hydrochloride

Selected References

[1] Ueda et al. (2003) Current and prospective applications of metal ion–protein binding. Journal of Chromatography 988:1.