Immobilized metal affinity chromatography (IMAC) is most frequently used for the purification of polyhistidine (His-) tagged proteins. This technique is based on the interaction between certain exposed protein residues (preferentially histidines) with transition metal cation Ni2+. Ni2+ itself is immobilized to a cross-linked agarose matrix via a chelating group such as nitrilotriacetic acid (NTA).
Ferrimagnetic agarose beads suitable for fast & efficient small-scale purification of polyhistidine (His) tagged proteins.
6 % cross-linked agarose suitable for FPLC, gravity flow and batch purification by a one-step procedure from crude lysates both under denaturing and non-denaturing conditions
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