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Glutathione Agarose

Suitable for FPLC, gravity flow and batch purification

4% cross-linked Glutathione agarose is a superior agarose support with covalently coupled glutathione attached via a 12-atom spacer. Its unique properties facilitate the rapid and high-yielding one-step purification of some native Glutathione S Transferase (GST) enzymes and recombinant GST-tagged proteins. Furthermore, Glutathione agarose is suitable for GST-pull down assays.

  • Excellent binding capacity based on high surface area to volume ratio
  • Notable mechanical and chemical stability
  • Reusable without any significant decrease in yield

Glutathione Agarose characteristics

Matrix4 % agarose
Linker12-atom spacer
Bead size50 - 150 µm
Binding capacity* > 8 mg/ml resin
Max. linear flow rate250 cm/h
pH stability 2 - 12
Chemical stabilitystable in 0.1 M acetate pH 4.0, 0.1 M NaOH,
70 % ethanol or 6 M guanidine hydrochloride for two hours at room temperature,
not autoclavable
Recommended flow rates

bed volume

inner diameter

sample loading


1 ml

6.6 mm

0.2 - 1.0 ml/min

1.0 ml/min

10 ml

16 mm

0.5 - 5.0 ml/min

5.0 ml/min

* tested with human Glutathione S-transferase (GST)

Selected References

[1] Bee et al. (2010) Growth and tumor suppressor NORE1A is a regulatory node between Ras signaling and microtubule nucleation. J. Biol. Chem. 285 (21):16258.