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Purification of Glycosylated Proteins

Boronic acids can covalently react with cis-diol groups within the oligosaccharide chains of glycoproteins thereby forming five- or six-membered cyclic esters. Based on this interaction, m-Aminophenylboronic acid-Agarose might be suitable for the purification/enrichment of glycoproteins.

Selected References

[1] Wang et al. (2013) Boronic Acid-Based Approach for Separation and Immobilization of Glycoproteins and Its Application in Sensing. Int J Mol Sci. 14(10):20890.
[2] Miksík et al. (1997) Post-translational non-enzymatic modification of proteins. II. Separation of selected protein species after glycation and other carbonyl-mediated modifications. J. Chromatogr. B Biomed. Sci. Appl. 699(1):311.
[3] Peacock et al. (1984) Glycosylated haemoglobin: measurement and clinical use. J. Clin. Pathol.37(8):841.