| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| CLK-1036-1 | 1 ml | 218,40 | Add to Basket/Quote Add to Notepad |
For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at 4 °C
do not freeze
Shelf Life: 12 months after date of delivery
Form: 10 mg/ml suspension containing 0.05 % Kathon
Description:
Azide Magnetic Beads are an efficient magnetic matrix to covalently capture CLICK-functionalized proteins by a Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or a Cu(I)-free azide-DBCO click chemistry reaction. The proteins of interest need to be metabolically, enzymatically or chemically alknye-tagged (Cu(I)-catalyzed reaction) or DBCO-tagged (Cu(I)-free reaction). Subsequently, the azide magnetic beads containing the covalently attached proteins can be washed with high stringency, virtually eliminating any non-specifically bound proteins.
Azide Magnetic Beads are not stable in organic solvents and high urea concentrations. Use Azide Agarose(CLK-1038) for mass spectrometry experiments.
Degree of substitution: 30-50 nmol Azide-groups/mg resin
Surface area: 5.7 * 109 μm2/mg
Mean bead diameter: 0.97 μm
Density: 1.33 g/cm3
Polymere composition: benzene, ethyenyl- and homopolymer (encapsulated magnetite)
