The recombinant streptococcal Protein G contains only highly homologous IgG-binding domains. The binding site is located on the Fc region of immunoglobulin. The albumin-binding domain as well as cell wall and cell membrane binding domains, present in native Protein G, have been removed to ensure maximum specific IgG binding capacity. Protein G has affinity for IgG from a variety of mammalian species and for some IgA and IgM.
Protein G Agarose characteristics
|Matrix||4% cross-linked agarose|
|Bead size||50 - 150 µm|
|Binding capacity||~ 20 mg human IgG / ml resin|
|Ligand density||~ 3 mg Protein G / ml resin|
|Maximum flow rate||500 cm/h|
|Maximum pressure||1.5 bar (21.8 psi)|