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Protein G Agarose

The recombinant streptococcal Protein G contains only highly homologous IgG-binding domains. The binding site is located on the Fc region of immunoglobulin. The albumin-binding domain as well as cell wall and cell membrane binding domains, present in native Protein G, have been removed to ensure maximum specific IgG binding capacity. Protein G has affinity for IgG from a variety of mammalian species and for some IgA and IgM.

  • High IgG-binding capacity
  • Higly stable bound Protein G: resin is reusable with no significant loss of binding capacity

 

Protein G Agarose characteristics

Matrix4% cross-linked agarose
Bead size50 - 150 µm
Binding capacity~ 20 mg human IgG / ml resin
Ligand density~ 3 mg Protein G / ml resin
Maximum flow rate500 cm/h
Maximum pressure1.5 bar (21.8 psi)

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