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Antibody Immobilization - Protein A Agarose

Protein A is a cell wall component of Staphylococcus aureus. It consists of a single polypeptide chain shaped as a cylinder, which contains five antibody binding domains. These high affinity regions are specifically bound to the Fc region of immunoglobulins (IgGs).

Protein A is temperature stable and it retains its native conformation even in the presence of denaturing agents. Protein A resins have been widely used to purify a broad range of immunoglobulins from different mammalian species.

  • High IgG-binding capacity
  • Milder elution condition than Protein G resin
  • Highly stable bound Protein A: resin is reusable with no significant loss of binding capacity

 

Protein A Agarose characteristics

Matrix4% cross-linked agarose
Bead size50-150 µm
Binding capacity~ 25 mg human IgG / ml resin
Ligand density~ 3 mg rProtein A / ml resin
Linear flow rate500 cm/h
Maximum pressure1.5 bar (21.8 psi)