The ability to purify recombinant proteins using affinity chromatography has greatly advanced protein research.
In affinity chromatography, the protein of interest is purified by its ability to bind a specific ligand that is immobilized on a chromatographic bead material (matrix).
Crude cell-lysates are incubated with such a matrix under conditions that ensure specific binding of the protein to the immobilized ligand. Other proteins that do not bind the immobilized ligand are washed through. Elution of the bound protein of interest can be achieved by changing the experimental conditions to favour desorption.
Jena Bioscience offers both Agarose and Magnetic Beads as matrix for different purification tasks.
Click on the links below to visit the appropriate product sections:
| Purification of | Agarose | Magnetic Beads |
|---|---|---|
| Nucleotide-binding proteins | NTP/NDP/NMP Agaroses | |
| mRNA cap binding proteins | m7GTP Agarose | |
| ATP-binding proteins | ATP Agaroses ATP Affinity Test Kit ATP Affinity Purification Kits |
|
| AMP-binding proteins | AMP Agaroses AMP Affinity Test Kit |
|
| Poly(A)-binding proteins | Poly(A)-Agarose | |
| GST-tagged proteins | Glutathione Agarose | Glutathione Magnetic Beads |
| His-tagged proteins | Ni-NTA Agarose | Ni-NTA Magnetic Beads |
| CLICK-functionalized proteins | Azide Agarose Alkyne Agarose PC Alkyne Agarose DBCO Agarose |
Azide Magnetic Beads Alkyne Magnetic Beads DBCO Magnetic Beads |
| Glycosylated proteins | m-Aminophenylboronic acid |
