Human adenovirus 2, Strain Adenoid 6
native, Hep-2 cell culture
For in vitro use only!
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Culture system: Hep-2 cell culture
Protein concentration: Lot specific. Determined by Bradford assay (Biorad) using BSA as standard or by UV absorption.
Analysis: SDS-PAGE and ELISA
Preparation: Harvested infected cells are lysed, treated with solvent to remove cellular components and ultracentrifuged to remove whole virus particles. Adenovirus Type 2 Hexon antigen is purified from the centrifugation supernatant by ion exchange chromatography. The antigen is presented in 30 mM Bis-Tris buffer at pH 6.8 containing sodium chloride.
Inactivation: Whole infective virus particles are removed by ultracentrifugation and chromatography. Confirmed inactive by attempted growth under original culture conditions. Since no procedure can guarantee absolute sterility, the reagent should be handled with appropriate safety precautions.