» Sign in / Register

Versatile Peroxidase

from Bjerkandera adusta

Enzyme Commission (EC) Number:

Mn(II): hydrogen-peroxide oxidoreductase / diarylpropane: oxygen, hydrogen-peroxide oxidoreductase

Cat. No. Amount Price (EUR) Buy / Note
EN-203S 200 Units 151,70 Add to Basket/Quote Add to Notepad
EN-203L 5 x 200 Units 606,80 Add to Basket/Quote Add to Notepad

For in vitro use only!

Unit Definition: One unit is defined as the amount of enzyme that oxidizes 1.0 μmole of Mn(II) per minute at pH 4.5 and 25 °C.

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C

Shelf Life: 12 months

Form: Freeze-dried brown-coloured amorphous powder, no stabilizing agent added

Solubility: water

Versatile peroxidase (syn. hybrid peroxidase, manganese-lignin peroxidase) is a new ligninolytic enzyme, combining catalytic properties of manganese peroxidase (oxidation of Mn(II)), lignin peroxidase (Mn-independent oxidation of non-phenolic aromatic compounds) and plant peroxidase (oxidation of hydro-quinones and substituted phenols). The manganese peroxidase component catalyzes the oxidation of Mn(II) to Mn(III) by H2O2. The highly reactive Mn(III) is stabilized via chelation in the presence of dicarboxylic acid.

2 Mn(II) + 2 H+ + H2O2 → 2 Mn(III) + 2 H2O

The lignin peroxidase component catalyzes the oxidation of non-phenolic aromatic rings into aryl cation radicals by H2O2. Aryl cation radicals are unstable and undergo various subsequent reactions. A typical example is the oxidation of veratryl alcohol (3,4-dimethoxybenzyl alcohol) into veratryl aldehyde (3,4-dimethoxybenzyl aldehyde) via the intermediary formation of veratryl cation and benzyl radicals.

Veratryl alcohol + H2O2 → Veratraldehyde + 2 H2O

Reaction conditions:
Optimal reaction with Mn(II) at pH 4.5, with veratryl alcohol at pH 3.0, Temperature range 15 - 50 °C.

Possible substrates:
Mn(II), Veratryl alcohol

Selected References:
Karimi et al. (2009) Color remediation of chemimechanical pulping effluent using combination of enzymatic treatment and Fenton reaction. Desalination 249 (2):870.
Schachschal et al. (2011) Encapsulation of enzymes in microgels by polymerization/cross-linking in aqueous droplets. Colloid and Polymer Science 289:693.