RalGDS-RBDGST
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).
Description:
Ras-binding domain (RBD) of the human RalGDS, comprises amino acids 788-885 (97aa) of wild type. RalGDS (Ral-guanine nucleotide dissociation stimulator) is the guanine nucleotide exchange factor (GEF) for the Ras-related protein Ral. The GEF-activity of RalGDS is stimulated by binding of Ras. The GST-tag facilitates the protein`s application in typical GST pull down assays.
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 40 kDa
Accession number: NM_006266
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 30 mM Tris-HCl pH 7.5, 100 mM NaCl, 5 mM MgCl2 and 5 mM DTE)
Selected References:
Vetter et al. (1999) Structural and biochemical analysis of Raseffector signaling via RalGDS. FEBS Letters 451:175.
Murphy et al. (2002) Involvement of phosphatidylinositol 3-kinase, but not RalGDS, in TC21/R-Ras2-mediated transformation. J. Biol. Chem. 277:9966.
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Scientific citations for Jena Bioscience pr-365
1 scientific citations and 0 citation images found by Bioz.
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Ras guanyl nucleotide releasing protein 2 affects cell viability and cell-matrix adhesion in ECV304 endothelial cells.
Cell adhesion & migration, 2014-02-06 · PMID:23563504
.. thrice with excess lysis buffer, and bound proteins were eluted in LDS sample buffer.. Rap1 activation was examined using
RalGDS-RBDGST (Jena Bioscience ) and Cosmogel® GST-Accept (Nacalai Tesque).. Briefly, cell lysates (150 μg) and RalGDS-RBDGST (2 μg) were incubated for 1 h ..
