Rab5A
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).
Description:
Rab5A is a small GTPase that belongs to the Ras superfamily. Rab proteins play an important role in various aspects of membrane traffic, including cargo selection, vesicle budding, vesicle motility, tethering, docking, and fusion. The small Rab5A GTPase is localized to plasma membrane, clathrin coated vesicles, caveosomes and early endosomes. Rab5 is a key regulator of the early endocytic pathway and plays a role in early endosome fusion and caveolar vesicle targeting to early endosomes. Furtermore it is also implicated in EGF receptor activation and apoptotic cell engulfment.
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 24 kDa
Accession number: NP_073183
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 25 mM HEPES pH 7.2, 40 mM NaCl, 2 mM MgCl2, 10 μM GDP and 3 mM TCEP)
Activity: 100 pmol of protein can bind > 80 pmol of GDP.
Selected References:
Stenmark et al. (2001) The Rab GTPase family. Genome Biol. 2:30071.
Yamaguchi et al. (2002) A GDP/GTP exchange protein for the Rab3 small G protein family up-regulates a postdocking step of synaptic exocytosis in central synapses. Proc. Natl. Acad. Sci. USA. 99:14536.
Kitano et al. (2008) Imaging of Rab5 activity identifies essential regulators for phagosome maturation. Nature 453 (7192):24.
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Scientific citations for Jena Bioscience pr-184
2 scientific citations and 7 citation images found by Bioz.
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The G-Protein Rab5A Activates VPS34 Complex II, a Class III PI3K, by a Dual Regulatory Mechanism
Biophysical Journal, 2020-12-01 · PMID:33137306
.. MWCO centrifugal filter (4400 rpm) to a volume of 1 mL before nucleotide exchange.. To exchange
Rab5A into the desired nucleotide, ∼11-fold excess GTP or GDP (Jena Bioscience , Jena, Germany) was added to the concentrate as well as 10 mM ethylenediaminetetraacetic acid and allowed to incubate for 1.5 h at room temperature.. Afterward, 20 mM MgCl 2 was added to replace the lost magnesium during ethylenediaminetetraacetic .. -
A Homogenous Bioluminescent System for Measuring GTPase, GTPase Activating Protein, and Guanine Nucleotide Exchange Factor Activities
Assay and drug development technologies, 2015-10-01 · PMID:26167953
Recombinant proteins NF1-333 (containing residues 1,198–1,530 of NF1 protein), RhoA,
Rab5A , Ran, RCC1, and RapGAP (Rna1p, GAP for yeast ortholog of mammalian Ran GTPase) were obtained fromJena Biosciences GmbH (Jena, Germany), the Ras protein was obtained from Millipore (Billerica, MA), the Rheb protein and guanosine-S′ [(β,γ)-methylano] triphosphate (GMP-PCP) were obtained from Sigma-Aldrich (St. Louis, MO).
